This study aims to provide a systematic and comprehensive introduction to the basic and clinical research progress of immunosenescence, thus accelerating research on immunosenescence in related diseases and promoting the development of targeted therapy
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75 g of protein lysates were heated for 10 min at 95 C in Laemmli 6X SDS sample buffer (Boston BioProducts, # BP-111R) with 5% 2-mercaptoethanol (VWR Life Science #M131-100ml) and then ran on 420% Criterion TGX pre-cast gels (Bio-Rad #5671093)
Its important to use these products consistently for the best results
However, knocking out FAP expressing fibroblasts in mice did not suppress inflammation but reduced the extent of tissue damage, confirming the pathogenic role of FAP in arthritis
109 Thus, a ferroptosis inhibitor alone cannot be used to support the conclusion of ferroptosis
